Singles
2016 Challenger Ciudad de Guayaquil
Champion	Nicolás Kicker
Runner-up	Arthur De Greef
Score	6–3, 6–2

Gastão Elias was the defending champion but lost in the first round to Guilherme Clezar.

Nicolás Kicker won the title after defeating Arthur De Greef 6–3, 6–2 in the final.

Seeds

Draw

Finals

	Semifinals							Final
		Arthur De Greef	7	7
		Guido Andreozzi	5	5
									Arthur De Greef	3	2
									Nicolás Kicker	6	6
		Nicolás Kicker	6	6
	Q	João Pedro Sorgi	3	1

Top half

	First round							Second round							Quarterfinals							Semifinals
	1	G Elias	2	6	3
		G Clezar	6	3	6				G Clezar	6	2	3
		P Sousa	1r						A De Greef	4	6	6
		A De Greef	3													A De Greef	6	6
		E Struvay	2	0r												M Bourgue	4	2
		C Zampieri	6	0					C Zampieri	3	1
		M Bourgue	4	6	6				M Bourgue	6	6
	8	J Souza	6	0	2																		A De Greef	7	7
	4	C Berlocq	1	6	3r																		G Andreozzi	5	5
		A Ghem	6	4	5				A Ghem	6	6
	LL	M Linzer	6	5				LL	M Linzer	3	2
	Q	Bastián Malla	2	2r												A Ghem	4	4
		G Andreozzi	6	64	6											G Andreozzi	6	6
	WC	E Gómez	3	77	3				G Andreozzi	7	6
	WC	I Endara	78	6				WC	I Endara	5	2
	7	R Dutra Silva	66	2

Bottom half

	First round							Second round							Quarterfinals							Semifinals
	6	S Giraldo	5	6	3
	Q	DE Galán	7	3	6			Q	DE Galán	5	3
	Q	A Molteni	6	6				Q	A Molteni	7	6
		C Garin	4	3											Q	A Molteni	3	0r
		N Kicker	6	6												N Kicker	6	3
	WC	G Escobar	1	1					N Kicker	3	6	6
		A Velotti	2	3				9	E Escobedo	6	2	1
	9	E Escobedo	6	6																			N Kicker	6	6
	5	V Estrella Burgos	3	77	67																	Q	JP Sorgi	3	1
	Q	JP Sorgi	6	65	79			Q	JP Sorgi	6	1	6
	WC	R Quiroz	2	2					L Mayer	3	6	1
		L Mayer	6	6											Q	JP Sorgi	4	6	6
		A Falla	6	5	3										2	F Bagnis	6	4	4
		M Arévalo	4	7	6				M Arévalo	2	6	4
		J Hernández	7	64	4			2	F Bagnis	6	4	6
	2	F Bagnis	5	77	6

References

• Main Draw
• Qualifying Draw