48 Hours may refer to:

Film and television

• 48 Hrs., a 1982 American action comedy film, starring Nick Nolte and Eddie Murphy
• 48 Hours (TV program), an American news/documentary TV program that has aired on CBS since 1988
• "48 Hours" (Brooklyn Nine-Nine), a television episode
• "48 Hours" (Stargate SG-1), a television episode
• 48 Hour Film Project, a short-film competition
• 48Hours, a filmmaking competition in New Zealand
• The First 48, an American documentary TV program

Music

• 48 Hours (album), an unreleased album by Frank-N-Dank
• 48 Hours (song), a song by Blue System
• "48 Hours", a song by The Clash from the 1977 album The Clash
• "48 Hours", a song by the Vengaboys from the 2000 album The Platinum Album
• "48Hrs", a song by Disciples released in 2018

Other

• Surgères 48 Hour Race, an ultrarunning race (1985–2010)