Biffen is a surname. Notable people with the surname include:

• Charles Edward Biffen, fictional character in the short story The Rummy Affair of Old Biffy by P. G. Wodehouse
• John Biffen, PC, DL (1930–2007), Conservative member of the UK House of Lords, after 36 years in the House of Commons
• Rowland Biffen (1874–1949), British botanist, geneticist, mycologist, professor of agricultural botany at the University of Cambridge
• Sarah Biffen (1784–1850), Victorian English painter born with no arms

See also

• Biffen och Bananen (the Beef and the Banana), a comic strip by Rit-Ola, originally published in Folket i Bild, 1936–1978
• Biffen Lecture, lectureship organised by the John Innes Centre, named after Rowland Biffen