Greathouse is an English surname. Notable people with the surname include:
- Clarence Ridgley Greathouse (1846–1899), American lawyer, journalist and diplomat
- Daniel Greathouse (c.1752 – 1775), American pioneer
- Ed Greathouse (1899–1954), American boxer
- Shiloh Greathouse, American skateboarder
- William M. Greathouse (1919–2011), American Nazarene minister and theologian
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