Iscariot most commonly refers to Judas Iscariot, one of the Twelve Apostles best known for betraying Jesus.
Iscariot may also refer to:
- Judas Iscariot (band), an American black metal band
- Pisces Iscariot, a compilation album by American alternative rock band The Smashing Pumpkins
- Iscariot (Hellsing), a fictional demon-hunting organization in the anime and manga, Hellsing
- Iscariot, a 2008 Swedish thriller film directed by Miko Lazic
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