John Hope Johnstone may refer to:
- John Hope-Johnstone (1796–1876), Scottish Conservative MP, de jure 7th Earl of Annandale and Hartfell
- John Hope-Johnstone (1842–1912) (1841–1912), Scottish Conservative MP, de jure 8th Earl of Annandale and Hartfell
- John Hope-Johnstone (photographer) (1883–1970), British photographer, associated with the Bloomsbury Group
See also
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