John Jeffries (1745–1819) was a physician and scientist.
John Jeffries may also refer to:
- John Calvin Jeffries (born c. 1948), law professor
- John Jeffries II (1796–1876), American ophthalmic surgeon
- John Jeffries (judge) (1929-2019), former judge, local politician and senior civil servant
See also
- John Jeffries Award, awarded to Harry George Armstrong
- John Jeffreys (disambiguation)
- John Jeffrey (disambiguation)
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