KIFAP3
Identifiers
AliasesKIFAP3, FLA3, KAP-1, KAP-3, KAP3, SMAP, Smg-GDS, dJ190I16.1, kinesin associated protein 3
External IDsOMIM: 601836 MGI: 107566 HomoloGene: 7799 GeneCards: KIFAP3
Orthologs
SpeciesHumanMouse
Entrez

22920

16579

Ensembl

ENSG00000075945

ENSMUSG00000026585

UniProt

Q92845

P70188

RefSeq (mRNA)

NM_010629
NM_001305643

RefSeq (protein)

NP_001292572
NP_034759

Location (UCSC)Chr 1: 169.92 – 170.09 MbChr 1: 163.61 – 163.74 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Kinesin-associated protein 3 (KAP3) is a protein that in humans is encoded by the KIFAP3 gene.[5][6] It is a non-motor, accessory subunit which co-oligomerizes with the motor subunits KIF3A and KIF3B or KIF3C, to form heterotrimeric kinesin-2 motor proteins. Kinesin-2 KAP subunits were initially characterized in echinoderms and mice.[7][8]

Function

The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 Armadillo repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein.[6] It has also been proposed to act as a clamp, stabilizing the C-terminal half [9] of the otherwise unstable stalk coiled-coil.

Interactions

KIFAP3 has been shown to interact with APC,[10] SMC3[11] and RAP1GDS1.[5]

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000075945 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000026585 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. 1 2 Shimizu K, Kawabe H, Minami S, Honda T, Takaishi K, Shirataki H, Takai Y (Dec 1996). "SMAP, an Smg GDS-associating protein having arm repeats and phosphorylated by Src tyrosine kinase". J Biol Chem. 271 (43): 27013–7. doi:10.1074/jbc.271.43.27013. PMID 8900189.
  6. 1 2 "Entrez Gene: KIFAP3 kinesin-associated protein 3".
  7. ↑ Wedaman KP, Meyer DW, Rashid DJ, Cole DG, Scholey JM (February 1996). "Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex". J. Cell Biol. 132 (3): 371–80. doi:10.1083/jcb.132.3.371. PMC 2120715. PMID 8636215.
  8. ↑ Yamazaki H, Nakata T, Okada Y, Hirokawa N (August 1996). "Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B". Proc. Natl. Acad. Sci. U.S.A. 93 (16): 8443–8. Bibcode:1996PNAS...93.8443Y. doi:10.1073/pnas.93.16.8443. PMC 38690. PMID 8710890.
  9. ↑ Ahmed Z, Mazumdar S, Ray K (February 2020). "Kinesin associated protein, DmKAP, binding harnesses the C-terminal ends of the Drosophila kinesin-2 stalk heterodimer". Biochem Biophys Res Commun. 522 (2): 506–511. doi:10.1016/j.bbrc.2019.11.111. PMC 8407544. PMID 31784087.
  10. ↑ Jimbo T, Kawasaki Y, Koyama R, Sato R, Takada S, Haraguchi K, Akiyama T (April 2002). "Identification of a link between the tumour suppressor APC and the kinesin superfamily". Nat. Cell Biol. 4 (4): 323–7. doi:10.1038/ncb779. PMID 11912492. S2CID 10745049.
  11. ↑ Shimizu K, Shirataki H, Honda T, Minami S, Takai Y (March 1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide". J. Biol. Chem. 273 (12): 6591–4. doi:10.1074/jbc.273.12.6591. PMID 9506951.

Further reading


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.