The following highways are numbered 327:

Canada

• Manitoba Provincial Road 327
• Nova Scotia Route 327
• Quebec Route 327

China

• China National Highway 327

Costa Rica

• National Route 327

India

• National Highway 327 (India)

Japan

• Japan National Route 327

United States

• Arkansas Highway 327
• Florida State Road 327
• Georgia State Route 327
• Indiana State Road 327
• Iowa Highway 327 (former)
• Kentucky Route 327
• Louisiana Highway 327
• Maryland Route 327
• Montana Secondary Route 327
• New Mexico State Road 327
• New York:
  • New York State Route 327
  • County Route 327 (Erie County, New York)
• Ohio State Route 327
• Pennsylvania Route 327 (former)
• Puerto Rico Highway 327
• South Carolina Highway 327
• Tennessee State Route 327
• Texas:
  • Texas State Highway 327
  • Texas State Highway Spur 327
  • Farm to Market Road 327
• Virginia State Route 327