Route 333, or Highway 333, may refer to:

Australia

- Holdsworth Road

Canada

• Newfoundland and Labrador Route 333
• Nova Scotia Route 333
• Prince Edward Island Route 333
• Quebec Route 333

Costa Rica

• National Route 333

Israel

• Israel National Highway 333

Japan

• Japan National Route 333

United States

• Arkansas Highway 333
• County Road 333 (Liberty County, Florida)
• Georgia State Route 333 (former)
• Georgia State Route 333
• Iowa Highway 333
• Kentucky Route 333
• Louisiana Highway 333
• Maryland Route 333
• Minnesota State Highway 333
• Mississippi Highway 333
• New Mexico State Road 333
• New York:
  • New York State Route 333 (former)
  • County Route 333 (Erie County, New York)
  • County Route 333 (Steuben County, New York)
• Ohio State Route 333 (disambiguation)
• Pennsylvania Route 333
• South Carolina Highway 333
• Tennessee State Route 333
• Texas:
  • Texas State Highway 333 (former)
  • Texas State Highway Loop 333 (former)
  • Farm to Market Road 333
• Virginia State Route 333
• Wyoming Highway 333 (former)

Other areas:

• Puerto Rico Highway 333
• U.S. Virgin Islands Highway 333