The following highways are numbered 401:

Canada

• British Columbia Highway 401 (former)
• Manitoba Provincial Road 401
• Ontario Highway 401

Costa Rica

• National Route 401

Japan

• Japan National Route 401

Norway

• Norwegian County Road 401

Thailand

• Thailand Route 401

United States

• U.S. Route 401
• Florida:
  • Florida State Road 401
  • County Road 401 (Brevard County, Florida)
• Georgia State Route 401 (unsigned designation for Interstate 75)
• Kentucky Route 401
• Nevada State Route 401
• New York:
  • New York State Route 401 (former)
  • County Route 401 (Albany County, New York)
• North Carolina Highway 401 (former)
• Pennsylvania Route 401
  • Pennsylvania Route 401 Alternate Truck
• Puerto Rico Highway 401
• Rhode Island Route 401
• Virginia State Route 401
• Washington State Route 401