Lois Wilson may refer to:
- Lois W. also known as Lois Wilson (née Burnham) (1891–1988), American co-founder of Al-Anon and wife of Alcoholics Anonymous founder Bill W.
- Lois Wilson (actress) (1894–1988), actress in silent films
- Lois Miriam Wilson (born 1927), first female Moderator of the United Church of Canada, 1980–1982
- Lois Wilson (Doctors), a fictional character from the British soap opera Doctors
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