Makino (written: 牧野 or 槙野) is a Japanese surname. Notable people with the surname include:

• Akira Makino (牧野 明, 1922–2007)
• Anna Makino (牧野 アンナ, born 1971), Japanese singer
• Makino Chikashige (牧野 親成, 1607–1677), Japanese daimyō
• Fred Kinzaburo Makino (牧野 金三郎, 1877-1953), Japanese newspaper editor
• Makino Hideshige (牧野 英成, 1671–1741), Japanese daimyō
• Hiroko Makino (牧野 紘子, born 1999), Japanese swimmer
• Hiroshi Makino (born 1956), Japanese golfer
• Kazu Makino (カズ 牧野), Japanese musician
• Keisuke Makino (牧野 景輔, born 1969), Japanese footballer
• Maria Makino (牧野 真莉愛, born 2001), Japanese singer
• Masahiro Makino (マキノ 雅弘, 1908–1993), Japanese film director
• Mitsunori Makino (牧野 光則, born 1988), Japanese shogi player
• Naoki Makino (牧野 直樹, born 1976), Japanese footballer
• Makino Narisada (牧野 成貞, 1634–1712), Japanese daimyō
• Nina Makino (牧野 仁菜, born 2005), A member of the Japanese group NiziU and former actress
• Makino Nobuaki (牧野 伸顕, 1861–1949), Japanese statesman
• Makino Sadamichi (牧野 貞通, 1707–1749), Japanese daimyō
• Makino Sadanaga (牧野 貞長, 1733–1796), Japanese daimyō
• Satoru Makino (牧野 悟, born 1990), Japanese footballer
• Shigeru Makino (牧野 茂, 1928–1984), Japanese baseball player and coach
• Shinji Makino (牧野 真二, born 1976), Japanese footballer
• Shiro Makino (牧野 四郎, 1893–1945), Japanese general
• Shozo Makino (disambiguation), multiple people
• Makino Tadahiro (牧野 忠寛, 1741–1766), Japanese daimyō
• Makino Tadakatsu (牧野 忠毅, 1859–1918), Japanese daimyō
• Makino Tadakiyo (牧野 忠精, 1760–1831), Japanese daimyō
• Makino Tadakuni (牧野 忠訓, 1844–1875), Japanese daimyō
• Makino Tadamasa (牧野 忠雅, 1799–1858), Japanese daimyō
• Tadasuke Makino (牧野 任祐, born 1997), Japanese racing driver
• Makino Tadayuki (牧野 忠恭, 1824–1878), Japanese daimyō
• Takao Makino (牧野 京夫, born 1959), Japanese politician
• Tomitaro Makino (牧野 富太郎, 1862–1957), Japanese botanist
• Tomoaki Makino (槙野 智章, born 1987), Japanese footballer
• Tsuyoshi Makino (牧野 剛, 1945–2016), Japanese writer, critic and social activist
• Yui Makino (牧野 由依, born 1986), Japanese actress, voice actress, singer and pianist
• Yukio Makino (牧野 幸雄), Japanese sailor