Toshiro Mifune (1920–1997) was a Japanese actor who appeared in over 150 feature films.

Mifune may also refer to:

• Jiro Mifune (born 1972), game designer
• Mifune Chizuko (御船千鶴子) (1886–1911), female clairvoyant
• Mika Mifune (三船美佳) (born 1982), actress
• Kyuzo Mifune (三船久蔵) (1883–1965), judoka
• Captain Mifune, a character from The Matrix Revolutions
• Mifune (Soul Eater), a character in the manga and anime Soul Eater
• Admiral Mifune, a character from the anime The Irresponsible Captain Tylor
• Go Mifune, main character in the anime series Speed Racer
• Lord Mifune, a feudal ruler in Usagi Yojimbo
• General Mifune, a Samurai general in Naruto
• Mifune, a 1999 Danish film
• Mifune: The Last Samurai, 2015 American film
• Mifune, a town in Kamimashiki, Kumamoto, Japan.
• Mifune Group
• Mifune Dinosaur Museum
• Mifunesaurus, a dinosaur named after Toshiro Mifune

See also

• Mifune, Kumamoto (御船町; -machi), town in Kamimashiki District, Kumamoto, Japan
• Mifune's Last Song, a Dogme 95 film by Søren Kragh-Jacobsen