Musashino may refer to:

Geography

• Musashino (train), a train service in Japan
• Musashino, Tokyo, a city in Greater Tokyo, Japan
• Musashino Line, a railway line in Greater Tokyo, Japan
• Musashino Plateau, a tableland in the Kantō region of Japan

Space

• 3249 Musashino, an asteroid

In the arts

• Musashino (Utamaro), an ukiyo-e print set by Kitagawa Utamaro, c. 1798–99
• "Musashino", a short story by Doppo Kunikida
• Musashino! (AKA Musasi-no), the second season of the anime TV series Urawa no Usagi-chan
• Musashino Animation, a fictional Japanese animation studio and the setting of the anime TV series Shirobako