National road N-400
Carretera nacional N-400
Route information
Length	180 km (110 mi)
Major junctions
From	Toledo
To	Cuenca
Location
Country	Spain
Highway system
Highways in Spain Autopistas and autovías National Roads

The N-400 is a highway in Spain from Toledo to Cuenca. It is being upgraded to the Autovía A-40, section by section.

It starts at the junction of the N-401, and the Autovía A-42 east of Toledo.

It heads east to Aranjuez and the Autopista R-4 and Autovía A-4. It then heads east with a junction to the N-301. There is also a junction with the Autovía A-3 at Taracón.

The N-400 then heads through the Altos de Cabrejas to Cuenca and has been partly upgraded to the Autovía A-40.