NLF may stand for:
- National Liberation Front, name used by various independence groups, paramilitary organizations, and political parties in different countries
- Viet Cong, officially the National Liberation Front for South Vietnam, which existed from 1960 to 1977
- National Labour Federation (Pakistan)
- National Liberal Federation, organization of the British Liberal Party, 1877–1936
- National Labor Federation in Eretz-Israel, a national trade union center in Israel
- Natural laminar flow, an airfoil characteristic researched by Michimasa Fujino
- New Life Fellowship Church (Vietnam)
- Non-lactose fermenter, a type of bacterium that grows in Sorbitol-MacConkey agar
- Norwegian Airsport Federation, a multi-sport association that organizes hot air balloons, hang gliding, paragliding, and other ultra-light recreational aircraft.
- Nuclear Liabilities Fund, a UK body funding nuclear decommissioning
- Darnley Island Airport, IATA airport code "NLF"
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