Niccolai is an Italian surname. Notable people with the surname include:
- Armand Niccolai (1911–1988), American football player
- Comunardo Niccolai (born 1946), Italian footballer
- Giulia Niccolai (1934–2021), Italian photographer, poet, novelist and translator
See also
- Nicolai (disambiguation), German variant
- Nicolay (disambiguation), French variant
- Nikolai (disambiguation) or Nikolay, Slavic forms
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