Pioneer Conference may refer to:
- Pioneer Conference (Illinois), intercollegiate athletic conference that existed in the U.S. state of Illinois from 1947 to 1949
- Pioneer Conference (Indiana), high school athletic conference in the U.S. state of Indiana, founded in 2009
- Pioneer Conference (Ohio), high school athletic conference in the U.S. state of Ohio, founded in 1977
- Pioneer Conference (Texas), junior college athletic conference that existed in the U.S. state of Texas from 1949 to 1961
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