Queen of the Night may refer to:

Botany

• Queen of the night, Night-blooming cereus, several genera and species of cactus, especially:
  • Epiphyllum oxypetalum
  • Selenicereus grandiflorus
• Queen of the night, Cestrum nocturnum, a woody evergreen commonly known as night-blooming cestrum
• Reina de la noche ("queen of the night" in Spanish), Brugmansia, also called angel's trumpet

Archaeology

• Queen of the Night Relief, formerly known as the Burney Relief, a Mesopotamian terracotta plaque in the British Museum, who adopted the new name in 2003

Film, television, and theater

• The Queen of the Night, a 1994 Mexican film
• La Femme d'une nuit, a 1931 French film
• Queen of the Night (1931 German-language film) (Königin einer Nacht), a 1931 German film
• Queen of the Night (1931 Italian-language film) (La donna di una notte), a 1931 Italian film
• Queen of the Night (1951 film), a West German musical film
• Queen of the Night (2001 film) (Kraljica noći), a Croatian film
• Queen of the Night (2013 film), a South Korean film
• Queen of the Night (2014 film), Canadian film released as The Captive
• Queen of the Night, a 2013 immersive theater production at Sony Hall

Literature

• Queen of the Night, a 2010 novel by J. A. Jance
• The Queen of the Night, a 2016 novel by Alexander Chee

Music

• Queen of the Night, or Königin der Nacht, a major character in the 1791 Mozart opera The Magic Flute
  • "Queen of the Night aria" ("Der Hölle Rache kocht in meinem Herzen")
• Queen of the Night (Maggie Bell album)
• Queen of the Night (Loleatta Holloway album), 1978
• "Queen of the Night" (song), a 1993 single by Whitney Houston
• "Queen of the Night", a song by Angra from their EP Freedom Call
• "Queen of the Night", a song by Lovex from their 2011 album Watch Out!