St Luke's University may refer to:
- St. Luke's International University, formerly St. Luke's College of Nursing, a private university in Tokyo, Japan
- St. Luke's College of Nursing, Trinity University of Asia, in the Philippines
- St Luke's Campus of the University of Exeter, England
- St Luke's University, a fictional university in Bristol, England, in the Doctor Who UK tv series
See also
- St. Luke's University Health Network, group of hospitals in Pennsylvania, United States
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