The Book of Life may refer to:

• Book of Life, in the Jewish and Christian religions, a book in which God records the names of the righteous
• The Book of Life (Sinclair book), a 1921 book by Upton Sinclair
• The Book of Life (Harkness novel), the third of the All Souls trilogy by Deborah Harkness
• The Book of Life, a collection of short stories by Stuart Nadler
• The Book of Life: An Illustrated History of the Evolution of Life on Earth, a book edited by Stephen Jay Gould
• The Book of Life of the Doukhobors, 1909 only printed hymnal, a collection of oral hymns of the Doukhobors
• The Book of Life, a bonus CD of hits by Eric B & Rakim included with Rakim album The 18th Letter
• Book of Life (album), an album by Jamaican reggae artist I Wayne
• The Book of Life (1998 film), a 1998 film directed by Hal Hartley
• The Book of Life (2014 film), an American animated film directed by Jorge Gutierrez and produced by Guillermo del Toro, set against the Mexican Day of the Dead holiday
• The Book of Life: Daily Meditations with Krishnamurti, a collection of meditations gathered from lectures and writings of Jiddu Krishnamurti