Thylacine may refer to:
- Thylacine, largest known carnivorous marsupial of modern times commonly known as the Tasmanian tiger
- Thylacine (band), Australian alternative band
- Thylacine Darner, or scientifically Acanthaeschna victoria, species of dragonfly in family Aeshnidae and endemic to Australia
- Thylacine (album)
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