Transpeptidase may refer to:
- DD-transpeptidase, a bacterial enzyme that cross-links the peptidoglycan chains to form rigid cell walls
- Gamma-glutamyl transpeptidase, a liver enzyme
- D-glutamyl transpeptidase
- A protein-sorting transpeptidase (e.g. sortase), that cleaves a C-terminal sorting signal from its target protein(s) and then covalently attaches the remainder to the cell surface.
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