Washington University of may refer to:
- University of Mary Washington, a public liberal arts university in Fredericksburg, Virginia.
- University of Washington, a public research university in Seattle, Washington
- Washington and Lee University, a private liberal arts college in Lexington, Virginia
- Washington College, a former secondary school also known as Washington College of Science and Industry in Irvington, California
- Washington Medical College, a defunct college named Washington University of Baltimore for 23 years
- Washington University in St. Louis, a private research university in St. Louis, Missouri
- Washington University of Barbados, a defunct for-profit medical school
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