Washington University of may refer to:

• University of Mary Washington, a public liberal arts university in Fredericksburg, Virginia.
• University of Washington, a public research university in Seattle, Washington
• Washington and Lee University, a private liberal arts college in Lexington, Virginia
• Washington College, a former secondary school also known as Washington College of Science and Industry in Irvington, California
• Washington Medical College, a defunct college named Washington University of Baltimore for 23 years
• Washington University in St. Louis, a private research university in St. Louis, Missouri
• Washington University of Barbados, a defunct for-profit medical school