EcoRI
EcoRI（「I」是羅馬數字「1」）是一種核酸酶，來自某些特定品系的大腸桿菌（E. coli，也是其名稱由來），是此種細菌體內參與限制修飾系統的酵素。
EcoRI切位。
EcoRI分子晶體結構圖，左側是橙色與綠色部分是DNA鏈。
在分子生物學或其他分子層次的生物學研究上，EcoRI是一種常用的限制酶，可將特定序列的DNA切割成片段，切割後的小片段端點為5'端突出的黏狀末端。
參考文獻
- Bitinaite, J., D. A. Wah, Aggarwal, A. K., Schildkraut, I. (1998). "FokI dimerization is required for DNA cleavage." Proc Natl Acad Sci U S A 95(18): 10570-5.
- Pingoud, A., Jeltsch, A. (2001) Structure and function of type II restriction endonucleases, Nucl. Acids Res. 29. 18, 3705-3727.
- McClarin, J.A., Frederick, C.A., Wang, B.C., Greene, P., Boyer, H.W., Grable, J., Rosenberg, J.M. (1986) Structure of the DNA-EcoRI endonucleases recognition complex at 3 Å resolution, Science. 234. 4783, 1526-1542.
- Kurpiewski, M.R., Engler, L.E., Wozniak, L.A., Kobylanska, A., Koziolkiewicz, M., Stec, W.J, Jen-Jacobson, L. (2004) Mechanisms of coupling between DNA recognition and catalysis in EcoRI endonucleases, Structure 12. 1775-1788.
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