Water blue
Methyl blue

Aniline Blue WS, also called aniline blue, diphenylamine blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them.[1] It is a soluble dye used as a biological dye,[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.[4]

Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,[5] as well as to reveal callose structures in plant tissues.[6]

It can also be used in other connective tissue stains, such as Mallory's stain,[5] Gömöri trichrome stain, and Carstair's Method.[7] It is used in differential staining.

References

  1. ↑ "Stainsfile - Aniline blue WS". stainsfile.info. Archived from the original on 2019-02-09. Retrieved 2012-01-14.
  2. ↑ "Medical Definition of ANILINE BLUE". www.merriam-webster.com.
  3. ↑ "Fluorescence Microscope Images". Archived from the original on 2008-05-26. Retrieved 2012-01-14.
  4. ↑ "aniline blue".
  5. 1 2 "Stainsfile - Water blue". stainsfile.info. Archived from the original on 2012-02-07. Retrieved 2012-01-14.
  6. ↑ "Protocols - Staining with trypan blue and aniline blue - Felix Mauch's Group". Archived from the original on 22 June 2005.
  7. ↑ Carstairs, K. C. (1965). "The Identification of platelets and platelet antigens in histological sections". The Journal of Pathology and Bacteriology. 90 (1): 225-231.
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