Biotin PEG2 amine
Names
IUPAC name
N-{2-[2-(2-Aminoethoxy)ethoxy]ethyl}-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide
Identifiers
3D model (JSmol)
ChemSpider
UNII
  • InChI=1S/C16H30N4O4S/c17-5-7-23-9-10-24-8-6-18-14(21)4-2-1-3-13-15-12(11-25-13)19-16(22)20-15/h12-13,15H,1-11,17H2,(H,18,21)(H2,19,20,22)/t12-,13-,15-/m0/s1
    Key: LWISPDYGRSGXME-YDHLFZDLSA-N
  • InChI=1/C16H30N4O4S/c17-5-7-23-9-10-24-8-6-18-14(21)4-2-1-3-13-15-12(11-25-13)19-16(22)20-15/h12-13,15H,1-11,17H2,(H,18,21)(H2,19,20,22)/t12-,13-,15-/m0/s1
    Key: LWISPDYGRSGXME-YDHLFZDLBC
  • O=C1N[C@@H]2[C@@H](SC[C@@H]2N1)CCCCC(=O)NCCOCCOCCN
Properties
C16H30N4O4S
Molar mass 374.50 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Biotin PEG2 amine (biotine PEG2 amine) is a water-soluble pegylated biotin derivative used as linker in biotechnology and molecular biology applications.[1]

Uses

Biotin PEG2 amine is used as a linker[2] or cross linker.[3] This allows to attach specific compounds to proteins or to antibodies.

A common use of biotin PEG2 amine is to use EDC and crosslink the amine in the biotin PEG2 amine to carboxyl groups on protein residues that are either aspartate or glutamate or the carboxy-terminus of proteins.

A particular example of use is labeling red cells, which in turn allows for the detection of these labeled cells in small samples using flow cytometry.[4]

  • Biotin-PEG2-maleimide
  • Biotin-PEG3-amine
  • Biotin-PEG4-NHS

References

  1. ↑ "EZ-Link™ Amine-PEG2-Biotin".
  2. ↑ Bronfman, F.C.; et al. (2003). "Ligand-Induced Internalization of the p75 Neurotrophin Receptor: A Slow Route to the Signaling Endosome". The Journal of Neuroscience. 23 (8): 3209–3220. doi:10.1523/JNEUROSCI.23-08-03209.2003. PMC 6742322. PMID 12716928.
  3. ↑ Takashi Hara, Shin-ichi Matsuyama and Hajime Tokuda (2003). "Mechanism Underlying the Inner Membrane Retention of Escherichia coli Lipoproteins Caused by Lol Avoidance Signals". J. Biol. Chem. 278 (41): 40408–40414. doi:10.1074/jbc.m307836200. PMID 12896969.
  4. ↑ "The page cannot be found". Archived from the original on 2011-08-29. Retrieved 2012-04-25.
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