Bis-tris propane
Skeletal formula of bis-tris propane
Ball and stick model of bis-tris propane
Names
Preferred IUPAC name
2,2′-[Propane-1,3-diylbis(azanediyl)]bis[2-(hydroxymethyl)propane-1,3-diol]
Other names
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxymethyl)propane-1,3-diol]
Identifiers
3D model (JSmol)
1786109
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard 100.058.981
EC Number
  • 264-899-3
1734507
MeSH 1,3-bis(tris(hydroxymethyl)methylamino)propane
UNII
  • InChI=1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2 checkY
    Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N checkY
  • OCC(CO)(CO)NCCCNC(CO)(CO)CO
Properties
C11H26N2O6
Molar mass 282.337 g·mol−1
Appearance White crystals
Melting point 164 to 165 °C (327 to 329 °F; 437 to 438 K)
log P −2.794
Related compounds
Related compounds
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, also known as BTP, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pKa values which are close in value. This buffer is primarily used in biochemistry and molecular biology.

Applications

A review of DNA polymerase fidelity cites bis-tris propane as a suitable buffer for polymerase chain reaction (PCR).[1] Bis-Tris propane has also been used with HCl buffer for stabilization of farnesyl diphosphate isolated from a strain of Saccharomyces cerevisiae.[2] It has also been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodopsin.[3] Use of Bis-Tris propane has also been documented in an investigation of the MgATPase activity of the myosin subfragment 1 monomer.[4] The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane.[5] Bis-Tris propane wide buffering range is also useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria.[6] Bis-Tris propane has been used as the buffering agent in separation of full and empty capsids of recombinant adeno-associated virus vectors with anion-exchange chromatography.[7][8]

See also

References

  1. ↑ Eckert KA, Kunkel TA (August 1991). "DNA polymerase fidelity and the polymerase chain reaction". PCR Methods and Applications. 1 (1): 17–24. doi:10.1101/gr.1.1.17. PMID 1842916.
  2. ↑ Song L (June 2003). "Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants". Analytical Biochemistry. 317 (2): 180–185. doi:10.1016/S0003-2697(03)00138-6. PMID 12758256.
  3. ↑ Tóth-Boconádi R, Dér A, Keszthelyi L (June 2000). "Buffer effects on electric signals of light-excited bacteriorhodopsin". Biophysical Journal. 78 (6): 3170–3177. Bibcode:2000BpJ....78.3170T. doi:10.1016/S0006-3495(00)76853-6. PMC 1300898. PMID 10827993.
  4. ↑ Bachouchi N, Garrigos M, Morel JE (September 1986). "MgATPase activity of myosin subfragment 1. The dimer is more active than the monomer". Journal of Molecular Biology. 191 (2): 247–254. doi:10.1016/0022-2836(86)90261-5. PMID 2949083.
  5. ↑ Wenner JR, Bloomfield VA (March 1999). "Buffer effects on EcoRV kinetics as measured by fluorescent staining and digital imaging of plasmid cleavage". Analytical Biochemistry. 268 (2): 201–212. doi:10.1006/abio.1998.3079. PMID 10075809.
  6. ↑ Ivannikov MV, Macleod GT (June 2013). "Mitochondrial free Ca²⁺ levels and their effects on energy metabolism in Drosophila motor nerve terminals". Biophysical Journal. 104 (11): 2353–2361. Bibcode:2013BpJ...104.2353I. doi:10.1016/j.bpj.2013.03.064. PMC 3672877. PMID 23746507.
  7. ↑ Lock M, Alvira MR, Wilson JM (February 2012). "Analysis of particle content of recombinant adeno-associated virus serotype 8 vectors by ion-exchange chromatography". Human Gene Therapy Methods. 23 (1): 56–64. doi:10.1089/hgtb.2011.217. PMC 4015067. PMID 22428980.
  8. ↑ Khatwani SL, Pavlova A, Pirot Z (June 2021). "Anion-exchange HPLC assay for separation and quantification of empty and full capsids in multiple adeno-associated virus serotypes". Molecular Therapy: Methods & Clinical Development. 21: 548–558. doi:10.1016/j.omtm.2021.04.003. PMC 8099603. PMID 33997103.
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