Countercurrent distribution (CCD, also spelled "counter current" distribution) is an analytical chemistry technique which was developed by Lyman C. Craig in the 1940s.[1] Countercurrent distribution is a separation process that is founded on the principles of liquid–liquid extraction where a chemical compound is distributed (partitioned) between two immiscible liquid phases (oil and water for example) according to its relative solubility in the two phases. The simplest form of liquid-liquid extraction is the partitioning of a mixture of compounds between two immiscible liquid phases in a separatory funnel.[2] This occurs in five steps: 1) preparation of the separatory funnel with the two phase solvent system, 2) introduction of the compound mixture into the separatory funnel, 3) vigorous shaking of the separatory funnel to mix the two layers and allow for mass transfer of compounds in and out of the phases, 4) The contents of the separatory funnel are allowed to settle back into two distinct phases and 5) the two phases are separated from each other by draining out the bottom phase. If a compound is insoluble in the lower phase it will distribute into the upper phase and stay in the separatory funnel. If a compound is insoluble in the upper phase it will distribute into the lower phase and be removed from the separatory funnel. If the mixture contains one or more compounds that are soluble in the upper phase and one or more compounds that are soluble in the lower phase, then an extraction has occurred. Often, an individual compound is soluble to a certain extent in both phases and the extraction is, therefore, incomplete. The relative solubility of a compound in two phases is known as the partition coefficient.

While one separatory funnel is useful in separating certain compound mixtures with a carefully formulated biphasic solvent system, a series of separatory funnels may be employed to separate compounds that have different partition coefficients. Countercurrent distribution, therefore, is a method of using a series of vessels (separatory funnels) to separate compounds by a sequence of liquid-liquid extraction operations. Contrary to liquid-liquid extraction, in the CCD instruments the upper phase is decanted from the lower phase once the phases have settled. First, a mixture is introduced to vessel 1 (V1) charged with both phases and the liquid-liquid extraction process is performed. The upper phase is added to a second vessel (V2) which already holds fresh lower phase. Fresh upper phase is added to V1. Both vessels are shaken and allowed to settle. upper phase from V1 is transferred to V2 at the same time the upper phase from V2 is transferred to V3 which already holds fresh lower phase. Fresh upper phase is added to V1, all three vessels are shaken and settled and the process continues.[3] Compounds that are more soluble in the upper phase than lower phase faster and farther down the series of vessels (the "train") while those compounds which are more soluble in the lower phase than the upper phase tend to lag behind. A compound insoluble in the upper phase will remain in V1 while a compound insoluble in the lower phase will stay in the lead vessel.

Historical development

Early work in the development of liquid-liquid separation techniques was undertaken by Cornish et al. with a process called "systematic fractional distribution"[4] as well as Randall and Longtin,[5] however, the central figure is certainly Lyman C. Craig. Lyman Craig's development of countercurrent distribution began with studying the distribution of a pharmaceutical, mepacrine (atabrine), between the two layers of an ethylene dichloride, methanol, and aqueous buffer biphasic solvent system.[6] The distribution coefficient (Kc which coincides with partition coefficient) of atabrine varied by the composition of the solvent system and the pH of the buffer. In the next article, Craig was inspired by the work of Martin and Synge with partition chromatography to develop an apparatus that would separate compounds based on their distribution constant (K which coincides with partition coefficient). It was shown that a solvent system composed of benzene, n-hexane, methanol and water would separate mixtures of organic acids.[7] It is remarkable that the mathematical theory developed hand-in-hand with the progression of applications.[8][9] Craig continued to pursue this method of separation by testing different compounds,[10] formulating biphasic solvent systems,[11] and most importantly developing a commercially viable instrument.[12][13]

The CCD technique was employed in many notable separations such as penicillin,[14] polycyclic aromatic hydrocarbons,[15] insulin,[16] bile acids,[17] ribonucleic acids,[18] taxol,[19] Streptomyces antibiotics.[20] and many other antibiotics.[21]

References

  1. ↑ Moore, Stanford (1978). "Lyman Creighton Craig 1906-1974". National Academy of Sciences Biographical Memoirs: 49–77. Retrieved 2016-02-26.
  2. ↑ Joseph-Nathan, P. (1967). "Liquid-liquid extraction". Journal of Chemical Education. 44 (3): 176. Bibcode:1967JChEd..44..176J. doi:10.1021/ed044p176.
  3. ↑ "Countercurrent Separations". 2 December 2013.
  4. ↑ Cornish, R. E.; Archibald, R. C.; Murphy, Elizabeth A.; Evans, H. M. (1934). "Purification of Vitamins - Fractional Distribution between Immiscible Solvents". Industrial & Engineering Chemistry. 26 (4): 397–406. doi:10.1021/ie50292a010.
  5. ↑ Randall, Merle; Longtin, Bruce (1938). "Separation Processes: General Method of Analysis". Industrial & Engineering Chemistry. 30 (9): 1063–1067. doi:10.1021/ie50345a028.
  6. ↑ Lyman C. Craig (1943). "Identification of Small Amounts of Organic Compounds by Distribution Studies. Application to Atabrine". Journal of Biological Chemistry. 150: 33–45. doi:10.1016/S0021-9258(18)51248-5.
  7. ↑ Lyman C. Craig (1944). "Identification of Small Amounts of Organic Compounds by Distribution Studies. II. Separation by Counter-current Distribution". Journal of Biological Chemistry. 155: 535–546.
  8. ↑ Williamson, Byron; Craig, Calvin (1947). "Identification of Small Amounts of Organic Compounds by Distribution Studies. V. Calculation of Theoretical Curves". Journal of Biological Chemistry. 168 (2): 687–697. doi:10.1016/S0021-9258(17)30926-2. PMID 20238623.
  9. ↑ Craig, L. C. (1950). "Partition Chromatography and Countercurrent Distribution". Analytical Chemistry. 22 (11): 1346–1352. doi:10.1021/ac60047a003.
  10. ↑ Sato, Yoshio; Barry, Guy T.; Craig, Lyman C. (1947). "Identification of Small Amounts of Organic Compounds by Distribution Studies. VII. Separation and Estimation of Normal Fatty Acids". Journal of Biological Chemistry. 170 (2): 501–507. doi:10.1016/S0021-9258(17)30832-3.
  11. ↑ Craig, Lyman C.; Golumbic, Calvin; Mighton, Harold; Titus, Elwood (1945). "Identification of Small Amounts of Organic Compounds by Distribution Studies. III. The Use of Buffers in Counter-current Distribution". Journal of Biological Chemistry. 161: 321–332. doi:10.1016/S0021-9258(17)41546-8. PMID 21005739.
  12. ↑ Craig, L. C.; Post, Otto (1949). "Apparatus for Countercurrent Distribution". Analytical Chemistry. 21 (4): 500–504. doi:10.1021/ac60028a013.
  13. ↑ Craig, L. C.; Hausmann, Werner; Ahrens, E. H.; Harfenist, E. J. (1951). "Automatic Countercurrent Distribution Equipment". Analytical Chemistry. 23 (9): 1236–1244. doi:10.1021/ac60057a009.
  14. ↑ Craig, Lyman C.; Hogeboom, George H.; Carpenter, Frederick H.; Vigneaud, Vincent du (1947). "Separation and Characterization of Some Penicillins by the Method of Counter-Current Distribution". Journal of Biological Chemistry. 168 (2): 665–686. doi:10.1016/S0021-9258(17)30925-0. PMID 20238622.
  15. ↑ Golumbic, Calvin. (1950). "Separation and Analysis of Polynuclear Compounds by Countercurrent Distribution". Analytical Chemistry. 22 (4): 579–582. doi:10.1021/ac60040a023.
  16. ↑ Harfenist, Elizabeth J.; Craig, Lyman C. (1951). "Countercurrent Distribution of Insulin". Journal of the American Chemical Society. 73 (2): 877–878. doi:10.1021/ja01146a538.
  17. ↑ Ahrens, Jr., Edward H.; Craig, Lyman C. (1952). "The Extraction and Separation of Bile Acids". Journal of Biological Chemistry. 195 (2): 763–778. doi:10.1016/S0021-9258(18)55787-2. PMID 14946188.
  18. ↑ Kirby, K.S. (1960). "Fractionation of ribonucleic acids by countercurrent distribution". Biochimica et Biophysica Acta. 41 (2): 338–340. doi:10.1016/0006-3002(60)90018-4. PMID 14409277.
  19. ↑ Wani, Mansukh C.; Horwitz, Susan Band (2014). "Nature as a remarkable chemist: a personal story of the discovery and development of Taxol". Anti-Cancer Drugs. 25 (5): 482–487. doi:10.1097/CAD.0000000000000063. PMC 3980006. PMID 24413390.
  20. ↑ Swart, E. Augustus. (1949). "The Use of Counter-Current Distribution for the Characterization of Streptomyces Antibiotics". Journal of the American Chemical Society. 71 (8): 2942–2944. doi:10.1021/ja01176a524.
  21. ↑ Craig, Lyman C.; Sogn, John (1975). "Isolation of antibiotics by countercurrent distribution". Methods in Enzymology. Vol. 43. Elsevier. pp. 320–346. doi:10.1016/0076-6879(75)43092-0. ISBN 978-0-12-181943-9. PMID 1134363.
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