De Beer is a Dutch and Afrikaans surname, meaning "the bear". Notable people with the surname include:

• Cornelius de Beer (c.1590–1651), Dutch painter and engraver active in Spain; son of Joos
• Dylan de Beer (born 1982), Zimbabwean cricketer
• Esmond Samuel de Beer (1895–1990), New Zealand bibliophile, art collector and philanthropist
• Fritz de Beer (born 1996), South African cricketer
• Gavin de Beer (1899–1972), British evolutionary embryologist
• Gerhard de Beer (born 1994), South African football player
• Hans de Beer (born 1957), Dutch illustrator and author
• Jan de Beer (c.1475–1528), Flemish painter and draughtsman
• Jannie de Beer (born 1971), South African rugby player
• Johan de Beer (born 1972), South African tennis player
• Joos de Beer (<1535–1591), Dutch painter
• Lonell de Beer (born 1980), South African cricketer
• Lotte de Beer, Dutch opera director
• María Eugenia de Beer (died 1652), Spanish engraver; daughter of Cornelius
• Racheltjie de Beer (1831–1843), Afrikaner heroine who may be fictional
• Roman de Beer (born 1994), South African racing driver
• Sam de Beer (born 1944), South African politician
• Sue de Beer (born 1973), American artist
• Surina De Beer (born 1978), South African tennis player
• Tinus de Beer (born 1996), South African rugby player
• Walter Wolff de Beer (1892–1983), Dutch philatelist
• Willem de Beer (born 1988), South African sprinter
• Willy de Beer (born 1942), Dutch speed skater
• Wolfgang de Beer (born 1964), German footballer
• Zach de Beer (1928–1999), South African politician and businessman

See also

• De Beers, diamond company named after the farmers Diederik Arnoldus and Johannes Nicolaas de Beer who sold the land with one of the first diamond mines of the company
• DeBeer Lacrosse, American sports equipment manufacturer