Lactobacillus delbrueckii subsp. lactis
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Bacillota
Class: Bacilli
Order: Lactobacillales
Family: Lactobacillaceae
Genus: Lactobacillus
Species:
L. delbrueckii
Subspecies:
L. d. subsp. lactis
Trinomial name
Lactobacillus delbrueckii subsp. lactis
(Orla-Jensen 1919) Rogosa & Hansen 1971 Weiss et al. 1984

Lactobacillus delbrueckii subsp. lactis is a subspecies of Lactobacillus delbrueckii that is generally used to measure the amount of cobalamin in food.[1] Its growth rate is proportional to the amount of cobalamin in the growth medium. However, lactis has been demonstrated to have the option to utilize pseudocobalamin, which is inactive for humans,[2] as well as "alkali-resistant factors" (deoxyribosides and deoxynucleotides),[1] leading to an overestimation of the amount of cobalamine in food.[3][4] As such, new methods using HPTLC[5] or LC-MS/MS[6] have also been developed.

References

  1. 1 2 Hoffmann CE, Stokstad EL, Hutchings B, Dornbush AC, Jukes TH (December 13, 1949). "The microbiological assay of vitamin B12 with Lactobacillus leichmannii". Journal of Biological Chemistry. 181 (2): 635–644. doi:10.1016/s0021-9258(18)56584-4. PMID 15393782. S2CID 35744983.
  2. ↑ Berman D, Yacowitz H, Weiser HH (January 1956). "A differential microbiological assay for vitamin B12 and pseudo vitamin B12". Applied Microbiology. 4 (1): 49–52. doi:10.1128/am.4.1.49-52.1956. PMC 1057156. PMID 13283540.
  3. ↑ Miyamoto E, Kittaka-Katsura H, Adachi S, Watanabe F (2005). "Assay of vitamin B12 in edible bamboo shoots". Vitamins. 79: 329–332.
  4. ↑ Watanabe F, Schwarz J, Takenaka S, Miyamoto E, Ohishi N, Nelle E, et al. (September 13, 2012). "Characterization of vitamin B₁₂compounds in the wild edible mushrooms black trumpet (Craterellus cornucopioides) and golden chanterelle (Cantharellus cibarius)". Journal of Nutritional Science and Vitaminology. 58 (6): 438–441. doi:10.3177/jnsv.58.438. PMID 23419403.
  5. ↑ Bito T, Yabuta Y, Watanabe F (October 1, 2013). "Miniaturized HPTLC of Vitamin B12 Compounds in Foods". Chromatographia. 76 (19): 1333–1337. doi:10.1007/s10337-013-2386-x. S2CID 97910208.
  6. ↑ Alsberg T, Minten J, Haglund J, Törnqvist M (December 30, 2001). "Determination of hydroxyalkyl derivatives of cobalamin (vitamin B12) using reversed phase high performance liquid chromatography with electrospray tandem mass spectrometry and ultraviolet diode array detection". Rapid Communications in Mass Spectrometry. 15 (24): 2438–2445. Bibcode:2001RCMS...15.2438A. doi:10.1002/rcm.527. PMID 11746915.
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