MBD3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2MB7
Identifiers
Aliases	MBD3, methyl-CpG binding domain protein 3
External IDs	OMIM: 603573 MGI: 1333812 HomoloGene: 2917 GeneCards: MBD3
Gene location (Human) Chr. Chromosome 19 (human)[1] Band 19p13.3 Start 1,573,596 bp[1] End 1,592,865 bp[1]
Gene location (Mouse) Chr. Chromosome 10 (mouse)[2] Band 10 C1|10 39.72 cM Start 80,228,373 bp[2] End 80,235,384 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in cingulate gyrus amygdala anterior pituitary left uterine tube Brodmann area 10 right lobe of thyroid gland gastrocnemius muscle Brodmann area 9 ganglionic eminence left lobe of thyroid gland Top expressed in lip yolk sac superior frontal gyrus neural tube entorhinal cortex ganglionic eminence esophagus thymus ankle joint abdominal wall More reference expression data BioGPS More reference expression data
Gene ontology Molecular function methyl-CpG binding DNA binding nucleosomal DNA binding RNA polymerase II cis-regulatory region sequence-specific DNA binding protein binding chromatin binding histone deacetylase activity Cellular component nucleus nucleoplasm heterochromatin chromosome NuRD complex cytoplasm chromatin protein-containing complex Biological process response to estradiol tissue development embryonic organ development regulation of DNA methylation in utero embryonic development response to organic cyclic compound regulation of transcription, DNA-templated heart development DNA methylation-dependent heterochromatin assembly histone acetylation negative regulation of transcription by RNA polymerase II human ageing brain development response to nutrient levels transcription, DNA-templated regulation of signal transduction by p53 class mediator histone deacetylation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 53615 17192 Ensembl ENSG00000071655 ENSMUSG00000035478 UniProt O95983 Q9Z2D8 RefSeq (mRNA) NM_003926 NM_001281453 NM_001281454 NM_013595 NM_001306143 RefSeq (protein) NP_001268382 NP_001268383 NP_001293072 NP_038623 Location (UCSC) Chr 19: 1.57 – 1.59 Mb Chr 10: 80.23 – 80.24 Mb PubMed search [3] [4]
Wikidata
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Methyl-CpG-binding domain protein 3 is a protein that in humans is encoded by the MBD3 gene.^[5][6][7]

Function

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA but instead binds to hydroxymethylated DNA.^[8] The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.^[7]

MBD3 also contains the coiled‐coil domain common to all three MBD3 isoforms. The coiled‐coil domain, but not the MBD domain, helps to maintain pluripotency of embryonic stem cells via the recruitment of polycomb repressive complex 2 to a subset of genes linked to development and organogenesis, thus establishing stable transcriptional repression.^[9]

Interactions

MBD3 has been shown to interact with:

• AURKA,^[10]
• GATAD2B,^[11][12]
• HDAC1,^[10][13][14]
• MTA2,^[10][13][14] and
• MBD2.^[13][15]

References

Further reading