2000s and founding

Metropoline was founded in 2000 by Tour Bus and two other transportation companies. In 2002, the company started to operate the intercity routes from BeerSheva to Tel Aviv and other destinations in the Southern District and the intracity service in Arad.

2010s

In 2010, Metropoline won the tender of Southern HaSharon which contained most intracity and regional service in Ramat HaSharon, Herzliya, Hod HaSharon, Ra'anana and Kfar Saba. In the same year Metropoline started to operate 7 routes that were transferred from Dan, and In early January 2011 Metropoline started to operate the routes that were transferred from Egged that were included in the tender. In 2012, Metropoline won again the tender of the Negev which contained all routes that Metropoline operated in the Southern District.

In July 2017, Metropoline won the tender of Sharon-Holon Regional which contains Netanya Tel Aviv routes were operated by Nateev Express, All Egged bus routes in Southern HaSharon, 4 bus routes of Dan in northern Tel Aviv and 4 bus routes of Egged in Holon.

In July 2018, Metropoline started to operate the Netanya Tel Aviv bus routes that were operated by Nateev Express.

In March 2019 Metropoline started to operate 3 bus routes that were operated by Egged with few additional new bus routes.

2020s

In March 2023, Metropoline won the tender of Ono Valley^{[lower-alpha 3]}-El'ad Regional which contains all routes that were operated by Kavim in the Ono Valley and El'ad, 3 bus routes of Dan in the same region, and all intercity routes from El'ad.^[2]

In June 2023, Metropoline began operating intracity and intrercity services in El'ad, as well as three lines from Ben Gurion Airport to various destinations in Gush Dan.

In October 2023, Metropoline began operating 8 routes in the Ono Valley region that were operated by Kavim. Metropoline will operate the rest of the lines in January 2024, as well as few lines from Dan and a new route.