o-Succinylbenzoate—CoA ligase
A cartoon representation of O-succinylbenzoate Co-A ligase of Staphylococcus aureus (strain N315). The different subunits are shown in different colors.
Identifiers
EC no.6.2.1.26
CAS no.72506-70-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

o-Succinylbenzoate—CoA ligase (EC 6.2.1.26), encoded from the menE gene in Escherichia coli, catalyzes the fifth reaction in the synthesis of menaquinone (vitamin K2). This pathway is called 1, 4-dihydroxy-2-naphthoate biosynthesis I.[1] Vitamin K is a quinone that serves as an electron transporter during anaerobic respiration. This process of anaerobic respiration allows the bacteria to generate the energy required to survive.

Background

The systematic name for the MenE enzyme is 2-succinylbenzoate: CoA ligase (AMP-forming). Other names for this enzyme include: o-succinylbenzoate-CoA synthase; o-succinylbenzoyl-coenzyme A synthetase; OSB-CoA synthetase; OSB: CoA ligase; synthetase, and o-succinylbenzoyle coenzyme A. The EC number is 6.2.1.26. MenE belongs to the ligase enzyme family, or class 6.

In the presence of 0.5mM of Ca(2+), K(+), Na(+), and Zn(2+) the enzyme activity was increased twofold. In the presence of .5 mM of Co(2+) and Mn(2+) the enzyme activity was increased fourfold. Mg(2+) is the ion that increases the enzyme activity the most. With .5 mM of Mg(2+) enzyme activity was increased sixfold. Inhibitors of this enzyme include diethylprocarbonate, Fe(2+), Hg(2+), and Mg(2+) (above 1mM).[2]

The maximum specific enzymatic activity is 3.2 micromol/min/mg. The optimum pH is 7.5. The maximum pH is 8. The optimum temperature is 30 degrees Celsius and the maximum temperature is 40 degrees Celsius. The molecular weight of o-succinylbenzoate CoA ligase is 185000 Da or 185 kDa. This enzyme is a tetramer, meaning it has four subunits in its quaternary structure.[2]

The PDB accession code is 3ipl. This is the crystal structure for o-succinylbenzoate CoA ligase in Staphylococcus aureus (strain N315) because the structure for E. coli has not been crystallized as of yet.[3]

Pathway

The pathway o-succinylbenzoate CoA ligase belongs to is called 1, 4-dihydroxy-2-napthoate biosynthesis I. Other organisms that contain this pathway are eukaryotic bacteria such as Bacillus anthracis.[4] Organisms that contain a pathway similar to this include Arabidopsis thaliana (gene AAE14),[5] Mycobacterium phlei,[6] and Synechocystis sp. (gene PCC 6803).[7] The reason for the difference in pathways is due to the varying functions of Vitamin K. Eukaryotic bacteria use vitamin K II while other organisms use vitamin K I. Other pathways that include o-succinylbenzoate CoA ligase include 1, 4-diydroxy-2-naphthoate biosynthesis II (i.e. in Arabidopsis thaliana), biosynthesis of secondary metabolites, metabolic pathways, ubiquinone, and other terpenoid-quinone biosynthesis. In Bacillus anthracis this enzyme is a target of potential antibiotic discovery.

Reaction

The reaction in vitamin K synthesis that includes MenE is as follows:

O-succinylbenzoate CoA ligase reaction in vitamin K synthesis.

ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 4-(2-carboxyphenyl)-4-oxobutanoyl-CoA

The substrates of this reaction are ATP, CoA, and 2-succinylbenzoate. The cofactors are ATP and CoA. The products are AMP, diphosphate, and 4-(2-carboxyphenyl)-4-oxobutanoyl-CoA.[8]

References

  1. ↑ van Oostende, Widhalm, Furt, Ducluzeau, Basset (2011). Phylloquinone (Vitamin K1): function, enzymes and genes. Advances in Botanical Research: Academic Press (Amsterdam). pp. 229–61.{{cite book}}: CS1 maint: multiple names: authors list (link)
  2. 1 2 Kwon O, Bhattacharyya DK, Meganathan R (December 1996). "Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and properties of o-succinylbenzoyl-coenzyme A synthetase from Escherichia coli". Journal of Bacteriology. 178 (23): 6778–81. doi:10.1128/jb.178.23.6778-6781.1996. PMC 178575. PMID 8955296.
  3. ↑ Patskovsky, Toro, Dickey, Sauder, Chang, Burley, Almo. "Crystal structure of o-succinylbenzoic acid-CoA ligase from Staphylococcus aureus". PDB. RCSB. Retrieved 6 December 2014.{{cite web}}: CS1 maint: multiple names: authors list (link)
  4. ↑ Tian Y, Suk DH, Cai F, Crich D, Mesecar AD (November 2008). "Bacillus anthracis o-succinylbenzoyl-CoA synthetase: reaction kinetics and a novel inhibitor mimicking its reaction intermediate". Biochemistry. 47 (47): 12434–47. doi:10.1021/bi801311d. PMC 2710618. PMID 18973344.
  5. ↑ Kim HU, van Oostende C, Basset GJ, Browse J (April 2008). "The AAE14 gene encodes the Arabidopsis o-succinylbenzoyl-CoA ligase that is essential for phylloquinone synthesis and photosystem-I function". The Plant Journal. 54 (2): 272–83. doi:10.1111/j.1365-313X.2008.03416.x. PMID 18208520.
  6. ↑ Sieweke HJ, Leistner E (August 1991). "o-Succinylbenzoate: coenzyme A ligase, an enzyme involved in menaquinone (vitamin K2) biosynthesis, displays broad specificity". Zeitschrift für Naturforschung C. 46 (7–8): 585–90. doi:10.1515/znc-1991-7-814. PMID 1663748. S2CID 12789671.
  7. ↑ Johnson, Naithani, Stewart Jr., Zybailov, Jones, Golbeck, Chitnis (6 March 2003). "The menD and menE homologs code for 2-succinyl-6-hydroxyl-2,4-cyclohexadiene-1-carboxylate synthase and O-succinylbenzoic acid-CoA synthase in the phylloquinone biosynthetic pathway of Synechocystis sp. PCC 6803". Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1557 (1–3): 67–76. doi:10.1016/S0005-2728(02)00396-1. PMID 12615349.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  8. ↑ Kolkmann R, Leistner E (December 1987). "4-(2'-Carboxyphenyl)-4-oxobutyryl coenzyme A ester, an intermediate in vitamin K2 (menaquinone) biosynthesis". Zeitschrift für Naturforschung C. 42 (11–12): 1207–14. doi:10.1515/znc-1987-11-1212. PMID 2966501. S2CID 41701934.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.