PAM
Identifiers
AliasesPAM, PAL, PHM, Peptidylglycine alpha-amidating monooxygenase
External IDsOMIM: 170270 MGI: 97475 HomoloGene: 37369 GeneCards: PAM
Orthologs
SpeciesHumanMouse
Entrez

5066

18484

Ensembl

ENSG00000145730

ENSMUSG00000026335

UniProt

P19021

P97467

RefSeq (mRNA)

NM_013626
NM_001357127

RefSeq (protein)

NP_038654
NP_001344056

Location (UCSC)Chr 5: 102.75 – 103.03 MbChr 1: 97.8 – 98.1 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Peptidyl-glycine alpha-amidating monooxygenase is an enzyme that catalyzes the conversion of glycine amides to amides and glyoxylate.

The enzyme is involved in the biosynthesis of many signaling peptides and some fatty acid amides.[5]

In humans, the enzyme is encoded by the PAM gene.[6][7] This transformation is achieved by conversion of a prohormone to the corresponding amide (C(O)NH2). This enzyme is the only known pathway for generating peptide amides, which renders the peptide more hydrophilic.[8]

Function

This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. The reaction pathway catalyzed by PAM is accessed via quantum tunneling and substrate preorganization.[9] Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene, but some of their full-length sequences are not yet known.[7]

The PHM subunit effects hydroxylation of an O-terminal glycine residue:

peptide-C(O)NHCH2CO2 + O2 + 2 [H] → peptide-C(O)NHCH(OH)CO2 + H2O

Involving hydroxylation of a hydrocarbon by O2, this process relies on a copper cofactor. Dopamine beta-hydroxylase, also a copper-containing enzyme, effects a similar transformation.[10]

The PAL subunit then completes the conversion, by catalyzing elimination from the hydroxylated glycine:

peptide-C(O)NHCH(OH)CO2 → peptide-C(O)NH2 + CH(O)CO2

The eliminated coproduct is glyoxylate, written above as CH(O)CO2.

In insects

Insect PαAMs are responsive to O2 concentrations and depends upon Cu2+. Simpson et al 2015 finds insect PαAMs to respond to hypoxia by regulating the activity of several peptide hormones. They find PαAM to probably be an important part of neuroendocrine responses to hypoxia.[11]

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000145730 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000026335 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Wilcox BJ, Ritenour-Rodgers KJ, Asser AS, Baumgart LE, Baumgart MA, Boger DL, et al. (March 1999). "N-acylglycine amidation: implications for the biosynthesis of fatty acid primary amides". Biochemistry. 38 (11): 3235–3245. doi:10.1021/bi982255j. PMID 10079066.
  6. Glauder J, Ragg H, Rauch J, Engels JW (June 1990). "Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells". Biochemical and Biophysical Research Communications. 169 (2): 551–558. doi:10.1016/0006-291X(90)90366-U. PMID 2357221.
  7. 1 2 "Entrez Gene: PAM peptidylglycine alpha-amidating monooxygenase".
  8. Eipper BA, Milgram SL, Husten EJ, Yun HY, Mains RE (April 1993). "Peptidylglycine alpha-amidating monooxygenase: a multifunctional protein with catalytic, processing, and routing domains". Protein Science. 2 (4): 489–497. doi:10.1002/pro.5560020401. PMC 2142366. PMID 8518727.
  9. McIntyre NR, Lowe EW, Belof JL, Ivkovic M, Shafer J, Space B, Merkler DJ (November 2010). "Evidence for substrate preorganization in the peptidylglycine α-amidating monooxygenase reaction describing the contribution of ground state structure to hydrogen tunneling". Journal of the American Chemical Society. 132 (46): 16393–16402. doi:10.1021/ja1019194. PMC 2988104. PMID 21043511.
  10. Abad E, Rommel JB, Kästner J (May 2014). "Reaction mechanism of the bicopper enzyme peptidylglycine α-hydroxylating monooxygenase". The Journal of Biological Chemistry. 289 (20): 13726–13738. doi:10.1074/jbc.M114.558494. PMC 4022847. PMID 24668808.
  11. Harrison JF, Greenlee KJ, Verberk WC (January 2018). "Functional Hypoxia in Insects: Definition, Assessment, and Consequences for Physiology, Ecology, and Evolution". Annual Review of Entomology. Annual Reviews. 63 (1): 303–325. doi:10.1146/annurev-ento-020117-043145. PMID 28992421.

Further reading

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