2000: two-year-old season

Regal Rose began her racing career at Ascot Racecourse on 28 July when she was one of eight fillies to contest a maiden race over six furlongs. Ridden by Johnny Murtagh, she started the 10/11 favourite ahead of Mujado who had finished second on her only previous start. She was restrained by Murtagh in the early stages before squeezing through a gap and moving into second behind Mujado in the last quarter mile. She began to show her lack of experience but finished strongly to take the lead 75 yards from the finish and won by three quarters of a length from Mujado with Velvet Glade in third.^[5]

After an absence of almost two months, Regal Rose was moved up sharply in class for the Group One Cheveley Park Stakes over six furlongs at Newmarket Racecourse on 26 September in which she was ridden by Frankie Dettori. Johnny Murtagh rode the stable's more fancied runner Enthused who started 11/8 favourite after wins in the Princess Margaret Stakes and the Lowther Stakes. Before the race Stoute explained that Enthused was a more precocious type whilst Regal Rose was likely to be better as a three-year-old.^[2] The Lowther Stakes runner-up Khulan was made second favourite on 9/2 with Regal Rose, despite her lack of experience being made the third choice in the betting on 11/2. The other ten runners included Sequoyah, the Aidan O'Brien-trained winner of the Moyglare Stud Stakes, Santolina (Sirenia Stakes), Lady Lahar (Futurity Stakes) and Autumnal (Windsor Castle Stakes). Regal Rose started slowly and fought against Dettori's attempts to restrain her as the 66/1 outsider Toroca set the pace. The filly began to make progress at half way and overtook Toroca approaching the final furlong. She hung slightly to the right in the closing stages but stayed on to win by a length, with Toroca getting the best of a four-way photo-finish for second ahead of Mala Mala, Autumnal and Sequoyah.^[6][7] After the race Dettori commented "She has a good cruising speed and an excellent turn of foot. With a faster pace she would have been more impressive".^[8]

2001: three-year-old season

As a three-year-old Regal Rose was aimed at the 1000 Guineas and was fancied for the race despite missing the trial races owing to the prevailing soft ground, but then sustained an injury to her left hind leg.^[9] It was hoped that the filly would return at Royal Ascot in June but she had further training problems, missed the race and was retired in August without running again. Cheveley Park's manager Chris Richardson commented "She had a lot of ability, but unfortunately had a setback in the spring and other niggling problems. We were hoping she would come back at first, but it was thought she would not perform back at the level we had hoped. She's an unbeaten Group 1 winner and it's disappointing we haven't seen her back, as we believe she was very good and wanted her to have the opportunity to reaffirm that".^[10]