Ru-Chih Chow Huang (Chinese: 黃周汝吉; pinyin: Huáng Zhōu Rǔjí; born 1932) is a Taiwanese-American biology professor at Johns Hopkins University. She is a biochemist who worked with James F. Bonner and Doug Fambrough to characterize and discern functions for nuclear histones in the early 1960s when the field lacked a consensus on types and functions of individual histone proteins.[1][2][3] Later she made discoveries about the molecular biology of cancer[4] and of viral gene regulation.[5]

Early life and education

Ru-Chih Chow was born April 2, 1932, in Nanjing, Jiangsu, China. She moved to the US in 1954. She was inspired to become a scientist after reading a biography of Marie Curie.[6] She received a BS from National Taiwan University in 1953, an MS from Virginia Tech in 1956, and her PhD from Ohio State University in 1960.[7] She was a postdoctoral fellow with James F. Bonner at California Institute of Technology from 1960 to 1965.[3]

Academic and research career

Ru-Chih Chow Huang joined the faculty at Johns Hopkins University in 1971.[6] She was the first female science professor hired there.[6] She was an Assistant Professor from 1965-1971, an Associate Professor from 1971-1975, and a Professor from 1975 to the present. Her title is McElroy Honorary Research Professor in 2018.[7] She served as Chairman of a Gordon Research Conference in 1980, Chairman of the Board of Science Counselors of the National Institute on Aging, National Institutes of Health, from 1980 to 1984, a member of the Board of Directors of the Institute of Molecular Biology, Taiwan from 1987 to 1988, and a member of the Science Advisory Board of the National Cancer Institute.[8]

In 1962, Huang and James Bonner published their finding that histone suppresses chromosomal RNA synthesis. That paper became a Citation Classic.[9] In the interview recognizing her paper as a Citation Classic, Huang said, "The work ... was done when little was known about the molecular approach to gene expression. The term chromatin as an interphase state of chromosome was beginning to be accepted as a biochemical working usage. The isolation and purification of chromatin was still approached with more art than science, until the pea embryo was chosen."[9] Bonner commented about the state of histone knowledge in the early 1960s when Huang began to work in his laboratory in a biographical article published in 1994, saying, "Study of the literature on histones made it clear that not much was known about how many kinds of histones there were or whether there were different histones in different creatures or in different specialized cells. Nobody knew what histones were for and no one had studied histones in plants."[3] Bonner got funding from NSF and other places to organize a conference on histones, trying to include all known histone researchers.[3] After the conference he summarized the overview they were left with as chaotic. Estimates of the number of histone types ranged up into the thousands and there was no consensus about homology of histones between different species or even between different cells within one species.[3]

Huang showed that histone proteins inhibited transcription of pea embryo and calf thymus DNA and worked out repeatable methods for studying individual histones while she was a postdoctoral fellow with Bonner.[2][3] Bonner's laboratory later purified and sequenced each histone type from peas in collaboration with Emil Smith.[3][1] In the years that followed at Johns Hopkins, her research focused upon gene regulation in cancer,[4] regulation of viral gene expression,[5] expression of viral genome fragments in mammalian genomes,[10] and molecular biology of aging cells.[11]

Huang is co-founder and Chief Scientific Advisor of  Erimos Pharmacueticals, LLC of Houston, TX. This firm is developing her recently discovered mutation-insensitive antiviral and anticancer compounds which are derivatives of the plant lignan NDGA.[8] This work was somewhat controversial when she first took it up.[6]

Personal life

Ru-Chih Chow married Pien Chien Huang on June 10, 1956. In an article in 2001, her son is quoted about her wedding day at Virginia Tech as follows, '"The same day she received her degree, she changed into a wedding dress in a Shell gas station in Blacksburg, Va., and they got married in the university president's office, with just the president and two witnesses,"said Suber Huang."They knew literally no one in this country. But they survived through their wit, intelligence and drive."'[6]

Awards

  • 1985, NIH-NSF Asian American Scientist Award
  • 2000, The World Journal Most Notable 100 North American Chinese of the Century[12]

References

  1. 1 2 RCC Huang and James Bonner. (1964) "Physical and biological properties of soluble nucleohistones."Journal of Molecular Biology 8 (1): 54-64.
  2. 1 2 R-C Huang and J Bonner (1962) "Histone, A Suppressor of Chromosomal RNA Synthesis" Proc Natl Acad Sci USA 48:1216-1222.
  3. 1 2 3 4 5 6 7 James Bonner (1994) "Chapters from my life." Annu Rev Plant Physiol. Plant Molecular Biol 45:1-23.
  4. 1 2 DE Hansel, S Dhara, RCC Huang, R Ashfaq, M Deasel, Y Shimada, HS Bernstein, J Harmon, M Brock, A Forastiere, MK Washington, A Maitra, E Montgomery (2005). "CDC2/CDK1 expression in esophageal adenocarcinoma and precursor lesions serves as a diagnostic and cancer progression marker and potential novel drug target." Am J Surg Pathol. 29:390-399.
  5. 1 2 IS Abd-Elazem, HS Chen, RB Bates, RCC Huang (2002). "Isolation of two highly potent and non-toxic inhibitors of human immunodeficiency virus type 1 (HIV-1) integrase from Salvia miltiorrhiza." Antiviral Res. 55:91-106.
  6. 1 2 3 4 5 Tom Pelton (2001). "Scientist, stung by criticism, is defiant." Baltimore Sun, December 16, 2001.
  7. 1 2 "Krieger School of Arts and Sciences, Johns Hopkins University". August 4, 2018. Retrieved August 12, 2018.
  8. 1 2 "Ru Chih Huang". Bloomberg. Retrieved August 14, 2018.
  9. 1 2 Citation Classics 12, March 20, 1978, RC Huang and J Bonner (1962) "Histone, a suppressor of chromosomal RNA synthesis." Proc. Natl. Acad. Sci. USA 48:1216-22, 1962.
  10. ↑ M Ono, MD Cole AT White, Ru Chih C Huang. (1980) “Sequence organization of clones intracisternal a particle genes.” Cell 21 (2):465-473.
  11. ↑ L Hoopes, A Brown, and Ru Chih C Huang. (1983) Methylation and rearrangement of mouse intracisternal a particle genes in development aging and myeloma.  Molecular and Cellular Biology 3 (8):1371-1380.
  12. ↑ "A Chinese Notable of the Century". JHU Gazette Online. Retrieved September 8, 2018.
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