Smash and Grab is the name given to a technique developed by Charles S. Hoffman and Fred Winston used in molecular biology to rescue plasmids from yeast transformants into Escherichia coli, also known as E. coli, in order to amplify and purify them.[1] In addition, it can be used to prepare yeast genomic DNA (and DNA from tissue samples) for Southern blot analyses or polymerase chain reaction (PCR).

References

  1. ↑ C.S. Hoffman; F. Winston (1987). "A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli". Gene. 57 (2–3): 267–72. doi:10.1016/0378-1119(87)90131-4. PMID 3319781.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.