Wilton Robinson Earle (June 22, 1902 – May 30, 1964) was an American cell biologist known for his research in cell culture techniques and carcinogenesis. Born in Greenville, South Carolina, he earned a bachelor's degree at Furman University then earned an M.A. at the University of North Carolina and PhD at Vanderbilt University in 1928. He joined the Hygienic Laboratory of the United States Public Health Service in 1928, which merged with the National Cancer Institute in 1937, where Earle worked the remainder of his life. He died at his home in Burtonsville, Maryland, aged 61.[1][2][3]

Career and research

Earle published or co-published more than one hundred scientific articles. He significantly contributed to the technology of the growth of cells in vitro.[1] He established the first continuous cell line derived from mouse fibroblast (L-cells)[4] and later also the first clonal cell line L929.[5][6] He formulated Earle's salts,[4] an isotonic saline solution (or balanced salt solution) with glucose and bicarbonate, which constitutes the base of many cell culture media.[7]

See also

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition [8]

Animal-cell culture media: History, characteristics, and current issues [9]

References

  1. 1 2 Evans, Virginia J. (1965). "Wilton R. Earle 1902–1964". In Vitro. 1 (1): vii. doi:10.1007/BF02618081. S2CID 9356715.
  2. ↑ "Dr. Wilton Earle, Biologist, 61, Dies". The New York Times. June 7, 1964.
  3. ↑ "Wilton Robinson Earle: 1902-1964". Journal of the National Cancer Institute. 35 (3): Suppl:3–13. 1965. PMID 5319645.
  4. 1 2 "Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells". JNCI: Journal of the National Cancer Institute. October 1943. doi:10.1093/jnci/4.2.165. ISSN 1460-2105.
  5. ↑ Sanford, K. K.; Earle, W. R.; Likely, G. D. (December 1948). "The growth in vitro of single isolated tissue cells". Journal of the National Cancer Institute. 9 (3): 229–246. ISSN 0027-8874. PMID 18105872.
  6. ↑ "NCTC clone 929 [L cell, L-929, derivative of Strain L] | ATCC". www.atcc.org. Retrieved 2022-02-07.
  7. ↑ Capes-Davis, Amanda (17 March 2021). Freshney's culture of animal cells : a manual of basic technique and specialized applications. John Wiley & Sons. ISBN 978-1-119-51304-9. OCLC 1163947979.
  8. ↑ Freshney, R. Ian (2010-09-20). Culture of Animal Cells. doi:10.1002/9780470649367. ISBN 9780470649367.
  9. ↑ Yao, Tatsuma; Asayama, Yuta (April 2017). "Animal-cell culture media: History, characteristics, and current issues". Reproductive Medicine and Biology. 16 (2): 99–117. doi:10.1002/rmb2.12024. PMC 5661806. PMID 29259457.


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