XPG N terminal domain
Crystal structure of flap endonuclease-1 r42e mutant from Pyrococcus horikoshii
Identifiers
SymbolXPG_N
PfamPF00752
Pfam clanCL0280
InterProIPR006085
PROSITEPDOC00658
SCOP21a77 / SCOPe / SUPFAM
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

In molecular biology the protein domain XPG refers to, in this case, the N-terminus of XPG. The XPG protein can be corrected by a 133 kDa nuclear protein, XPGC.[1] XPGC is an acidic protein that confers normal ultraviolet (UV) light resistance.[2] It is a magnesium-dependent, single-strand DNA endonuclease that makes structure-specific endonucleolytic incisions in a DNA substrate containing a duplex region and single-stranded arms.[2][3] XPGC cleaves one strand of the duplex at the border with the single-stranded region.[3]

Homology

XPG belongs to a family of proteins that includes:

  • RAD2 from Saccharomyces cerevisiae (Baker's yeast) and rad13 from Schizosaccharomyces pombe (Fission yeast), which are single-stranded DNA endonucleases,;[3][4]
  • mouse and human FEN-1, a structure-specific endonuclease;
  • RAD2 from fission yeast and RAD27 from budding yeast;
  • fission yeast exo1, a 5'-3' double-stranded DNA exonuclease that may act in a pathway that corrects mismatched base pairs;
  • yeast DHS1,
  • yeast DIN7.

Sequence alignment of this family of proteins reveals that similarities are largely confined to two regions. The first is located at the N-terminal extremity (N-region) and corresponds to the first 95 to 105 amino acids. The second region is internal (I-region) and found towards the C terminus; it spans about 140 residues and contains a highly conserved core of 27 amino acids that includes a conserved pentapeptide (E-A-[DE]-A-[QS]). It is possible that the conserved acidic residues are involved in the catalytic mechanism of DNA excision repair in XPG. The amino acid linking the N- and I-regions are not conserved.

Xeroderma pigmentosum

Xeroderma pigmentosum (XP) [1] is a human autosomal recessive disease, characterised by a high incidence of sunlight-induced skin cancer. People's skin cells with this condition are hypersensitive to ultraviolet light, due to defects in the incision step of DNA excision repair. There are a minimum of seven genetic complementation groups involved in this pathway: XP-A to XP-G. XP-G is one of the most rare and phenotypically heterogeneous of XP, showing anything from slight to extreme dysfunction in DNA excision repair.[2][5]

References

  1. 1 2 Tanaka K, Wood RD (February 1994). "Xeroderma pigmentosum and nucleotide excision repair of DNA". Trends Biochem. Sci. 19 (2): 83–6. doi:10.1016/0968-0004(94)90040-X. PMID 8160271.
  2. 1 2 3 O'Donovan A, Scherly D, Clarkson SG, Wood RD (June 1994). "Isolation of active recombinant XPG protein, a human DNA repair endonuclease". J. Biol. Chem. 269 (23): 15965–8. doi:10.1016/S0021-9258(17)33956-X. PMID 8206890.
  3. 1 2 3 O'Donovan A, Davies AA, Moggs JG, West SC, Wood RD (September 1994). "XPG endonuclease makes the 3' incision in human DNA nucleotide excision repair". Nature. 371 (6496): 432–5. Bibcode:1994Natur.371..432O. doi:10.1038/371432a0. PMID 8090225. S2CID 4328388.
  4. ↑ Habraken Y, Sung P, Prakash L, Prakash S (November 1993). "Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease". Nature. 366 (6453): 365–8. Bibcode:1993Natur.366..365H. doi:10.1038/366365a0. PMID 8247134. S2CID 4286568.
  5. ↑ Carr AM, Sheldrick KS, Murray JM, al-Harithy R, Watts FZ, Lehmann AR (March 1993). "Evolutionary conservation of excision repair in Schizosaccharomyces pombe: evidence for a family of sequences related to the Saccharomyces cerevisiae RAD2 gene". Nucleic Acids Res. 21 (6): 1345–9. doi:10.1093/nar/21.6.1345. PMC 309318. PMID 8464724.
This article incorporates text from the public domain Pfam and InterPro: IPR006085
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