Actinobacillus pleuropneumoniae
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Pseudomonadota
Class: Gammaproteobacteria
Order: Pasteurellales
Family: Pasteurellaceae
Genus: Actinobacillus
Species:
A. pleuropneumoniae
Binomial name
Actinobacillus pleuropneumoniae
(Pohl, 1983)

Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964.[1][2] It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii.[3]

Microbiology

Actinobacillus pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the family Pasteurellaceae.[4][5] It exhibits β-hemolysis activity,[6] thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD ('V factor') to facilitate growth for one of its biological variants (biovar 1).[3] As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow.[3] Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin.[3]

Porcine pleuropneumonia

Actinobacillus pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine.[3] The main disease associated with this bacterium is porcine pleuropneumonia, a highly contagious respiratory disease, affecting primarily young pigs (usually less than 6 months).[5] All of the symptoms and signs of porcine pleuropneumonia can be attributed to its virulence factors. The symptoms include respiratory distress, bloodstained discharge (usually frothy) from the mouth, fever, anorexia, mild diarrhea, cyanosis, lethargy, and spontaneous abortion in sows.[4][5] The most common sign for a pig farmer is the sudden death of several pigs over a short period of time.[4] Peak mortality is usually reached when pigs are 10–16 weeks old.[1] Not uncommonly,r mortality rates can reach 20-80% in fattening pigs, with similarly high morbidity.[1] Pigs that do survive the disease remain as carriers and spread the bacterium to other swine.[4] Several bacterial combinations are seen in vivo, the most common simultaneous infection being Pasteurella multocida.[3] Treatment must be immediate and continuous. Antibiotics used include ceftiofur, tetracycline, synthetic penicillins, tylosin, and sulfonamides.[7]

Serotypes

Nineteen different serotype variants (serovars) have been recognized for A. pleuropneumoniae,[8] based on the different capsular polysaccharides exhibited.[9] Two different biovars exist, with biovar 1 having 13 different serovars and biovar 2 having two serovars.[4] Differences in virulence potential, immunogenicity, and worldwide geographical distribution contribute to the diversity of the A. pleuropneumoniae serotypes.[9] All 15 serotypes can cause disease, with one serotype usually predominating in a particular herd.[10] The main difference between the serotypes is the expression of Apx toxins and other virulence factors.

Pathophysiology

The bacterium rapidly colonizes the host and attaches to the epithelial cells of the tonsils, moving down to the respiratory tract using type IV fimbriae.[4][10] As the bacteria replicate, they release cytotoxins (in the form of Apx toxins), hemolysins and the lipopolysaccharides (LPSs)on their outer membranes.[4] The subsequent lysis of macrophages causes a release of lysozymes, which in turn cause the tissue damage seen in porcine pleuropneumonia.[4] Members of the family Pasteurellaceae routinely change the cellular processes of the infected cell.[11] In particular, A. pleuropneumoniae activates the creation of various cytokines such as interleukin 1β (IL-1β), IL-8, and tumor necrosis factor-alpha (TNF-α).[11] IL-8 is itself a chemical signal used to attract neutrophils to the infection site.[11]

The typical presentation of A. pleuropneumoniae in pigs is the characteristic demarcated lesions in the middle, cranial, and caudal lobes of the lungs.[3] Areas of severe pneumonic growth are dark and consolidated.[3] In the case of chronically infected pigs, pleural adhesions and abscesses are normally found.[3] Histological studies of infected lung tissue normally showcase lung necrosis, neutrophil infiltration, macrophage and platelet activation, and an exudate.[3] Severe hemolysis or hemorrhaging is also present.

Several virulence factors account for the remarkable pathogenicity of A. pleuropneumoniae.[9] The more important ones include the production and release of the Apx toxins, the ability to produce a biofilm, its LPS layer, capsule polysaccharides, and its ability to survive within an iron-limited environment.[9] Of these, the most important are its capsule and Apx toxin production.[9]

The Apx toxin, a member of the RTX toxin family, is subdivided into four types: ApxI through ApxIV.[10] As a pore-forming exotoxin, Apx toxin lyses alveolar epithelial cells, endothelial cells, red blood cells, neutrophils, and macrophages.[10] Each serotype expresses different levelss of the four Apx toxins.[9] The most virulent combination known to exist, ApxI and ApxII, is expressed by serovars 1, 5, 9, and 11.[9] The ApxII and ApxIII combination is of medium virulence and is expressed by serovars 2, 3, 4, 6, 8, and 15.[9]

Epidemiology

The bacteria are usually spread through direct nose-to-nose contact.[1] It is species-specific, as its Apx toxin only affects pigs and other swine.[12] Overcrowding in pigpens, co-infections of other respiratory pathogens, and unusual stress all contribute to the spread of the disease.[1] A. pleuropneumoniae must have a host to survive, and does not survive for a significant time outside a host.[1] This bacterium is found worldwide, with different serotypes prevailing in different locations. Serotypes 1, 3, 5, and 7 are most commonly found in North America.[1]

Economic impact

A. pleuropneumoniae has a profound economic impact on pork production and pig farmers. In 1995, Its infections cost the U.S. economy about $30 million.[13] Such losses usually result from medication and veterinary expenses, increased mortality of pigs, extra labor, and other factors, such as reduced weight gain.[13]

References

  1. 1 2 3 4 5 6 7 "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 3 September 2018.
  2. ↑ Shope, RE (1964). "Porcine Contagious Pleuropnemonia: 1. Experimental Transmission, Etiology, and Pathology". Journal of Experimental Medicine. 119 (3): 357–368. doi:10.1084/jem.119.3.357. PMC 2137882. PMID 14129707.
  3. 1 2 3 4 5 6 7 8 9 10 Marsteller, TA; Fenwick B (1999). "Actinobacillus pleuropneumoniae disease and serology". Swine Health and Production. 7 (4): 161–165.
  4. 1 2 3 4 5 6 7 8 Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 April 2013.
  5. 1 2 3 Xu, Z; Chen X; Li L; et al. (2010). "Comparative Genomic Characterization of Actinobacillus pleuropneumoniae". Journal of Bacteriology. 192 (21): 5625–5636. doi:10.1128/JB.00535-10. PMC 2953695. PMID 20802045.
  6. ↑ Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma Health Sciences Center. Archived from the original on 13 October 2002. Retrieved 20 April 2013.
  7. ↑ Dee, S. "Pleuropneumonia in Pigs". Merck Veterinary Manual. Retrieved 20 April 2013.
  8. ↑ Stringer, O; Bosse J; Lacouture S (2021). "Proposal of Actinobacillus pleuropneumoniae serovar 19, and reformulation of previous multiplex PCRs for capsule-specific typing of all known serovars". Veterinary Microbiology. 255: 109021. doi:10.1016/j.vetmic.2021.109021. hdl:10044/1/86195. PMID 33667982. S2CID 232132425.
  9. 1 2 3 4 5 6 7 8 Klitgaard, K; Friis C; Angen O; et al. (2010). "Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential". BMC Genomics. 11: 698. doi:10.1186/1471-2164-11-698. PMC 3091793. PMID 21143895.
  10. 1 2 3 4 Chiers, K; De Waele T; Masmans F; et al. (2010). "Virulence factors of Actinobacillus pleuropneumoniae involved in colonization, persistence and induction of lesions in its porcine host". Veterinary Research. 41 (65): 65. doi:10.1051/vetres/2010037. PMC 2899255. PMID 20546697.
  11. 1 2 3 Auger, E; Deslandes V; Ramjeet M; et al. (2009). "Host-pathogen interactions of Actinobacillus pleuropneumoniae with porcine lung and tracheal epithelial cells". Infection and Immunity. 77 (4): 1426–1441. doi:10.1128/IAI.00297-08. PMC 2663157. PMID 19139196.
  12. ↑ Vanden Bergh, PGAC; Zecchinon LM; Fett T; et al. (2009). "Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity". Veterinary Research. 40 (33): 33. doi:10.1051/vetres/2009016. PMC 2701182. PMID 19356397.
  13. 1 2 Losinger, WC (2005). "Economic impacts of reduced pork production associated with the diagnosis of Actinobacillus pleuropneumoniae on grower/finisher swine operations in the United States". Preventive Veterinary Medicine. 68 (2–4): 181–193. doi:10.1016/j.prevetmed.2004.12.004. PMID 15820115.
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