EF手
EF手（英語：）是一种在钙结合蛋白中发现的螺旋-环-螺旋结构域或结构基序。
| EF手 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
 | |||||||||||
| 鑑定 | |||||||||||
| 標誌 | efhand | ||||||||||
| Pfam | PF00036（旧版） | ||||||||||
| InterPro | IPR002048 | ||||||||||
| PROSITE | PDOC00018 | ||||||||||
| SCOP | 1osa / SUPFAM | ||||||||||
| OPM蛋白 | 1djx | ||||||||||
| |||||||||||
EF手包括一个螺旋-环-螺旋拓扑结构，就像人类的手上拇指和食指，而Ca2+离子与其配体就位于环内。这一结构最早在小清蛋白中发现，小清蛋白含有三个EF手结构，与钙离子结合而造成的肌肉放松有关。
EF手包含两个α螺旋，由一个短的（通常包括12个氨基酸）环状区域连接，常用于结合钙离子。EF手也出现在钙调蛋白的所有结构域和肌肉蛋白质肌钙蛋白C中。
钙离子结合位点
分类
- 自从EF手结构模体在1973年被描绘以来，迄今为止该家族的蛋白质已扩展到至少66个亚家族。EF手结构基元可以分为Canonical EF-hands和Pseudo EF-hands 两大类：
EF手蛋白家族的系统发育树
亚族
- EPS15 homology (EH) domain – IPR000261
例子
人类基因组中，包含有EF手的蛋白质有：
- ACTN1、ACTN2、ACTN3、ACTN4、APBA2BP; AYTL1; AYTL2
- C14orf143; CABP1; CABP2; CABP3; CABP4; CABP5; CABP7; CALB1; CALB2; CALM2; CALM3; CALML3; CALML4; CALML5; CALML6; CALN1; CALU; CAPN1; CAPN11; CAPN2; CAPN3; CAPN9; CAPNS1; CAPNS2; CAPS; CAPS2; CAPSL; CBARA1; CETN1; CETN2; CETN3; CHP; CHP2; CIB1; CIB2; CIB3; CIB4; CRNN
- DGKA; DGKB; DGKG; DST; DUOX1; DUOX2
- EFCAB1; EFCAB2; EFCAB4A; EFCAB4B; EFCAB6; EFCBP1; EFCBP2; EFHA1; EFHA2; EFHB; EFHC1; EFHD1; EFHD2; EPS15; EPS15L1
- FKBP10; FKBP14; FKBP7; FKBP9; FKBP9L; FREQ; FSTL1; FSTL5
- GCA; GPD2; GUCA1A; GUCA1B; GUCA1C
- hippocalcin; HPCAL1; HPCAL4; HZGJ
- IFPS; ITSN1; ITSN2; KCNIP1; KCNIP2; KCNIP3; KCNIP4; KIAA1799
- LCP1
- MACF1; MRLC2; MRLC3; MST133; MYL1; MYL2; MYL5; MYL6B; MYL7; MYL9; MYLC2PL; MYLPF
- NCALD; NIN; NKD1; NKD2; NLP; NOX5; NUCB1; NUCB2
- OCM
- PDCD6; PEF1; PKD2; PLCD1; PLCD4; PLCH1; PLCH2; PLS1; PLS3; PP1187; PPEF1; PPEF2; PPP3R1; PPP3R2; PRKCSH; PVALB
- RAB11FIP3; RASEF; RASGRP; RASGRP1; RASGRP2; RASGRP3; RCN1; RCN2; RCN3; RCV1; RCVRN; REPS1; RHBDL3; RHOT1; RHOT2; RPTN; RYR2; RYR3
- S100A1; S100A11; S100A12; S100A6; S100A8; S100A9; S100B; S100G; S100Z; SCAMC-2; SCGN; SCN5A; SDF4; SLC25A12; SLC25A13; SLC25A23; SLC25A24; SLC25A25; SPATA21; SPTA1; SPTAN1; SRI
- TBC1D9; TBC1D9B; TCHH; TESC; TNNC1; TNNC2
- USP32
- VSNL1
- ZZEF1
另见
- 锚定结构域，另一种由α-螺旋组成的钙结合结构基序。
参考文献
- Ban C, Ramakrishnan B, Ling KY, Kung C, Sundaralingam M. . Acta Crystallogr. D Biol. Crystallogr. January 1994, 50 (Pt 1): 50–63. PMID 15299476. doi:10.1107/S0907444993007991.
延伸阅读
- Branden C, Tooze J. . . New York: Garland Pub. 1999: 24–25. ISBN 0-8153-2305-0.
- Nakayama S, Kretsinger RH. . Annu Rev Biophys Biomol Struct. 1994, 23: 473–507. PMID 7919790. doi:10.1146/annurev.bb.23.060194.002353.
- Zhou Y, Yang W, Kirberger M, Lee HW, Ayalasomayajula G, Yang JJ. . Proteins. November 2006, 65 (3): 643–55. PMID 16981205. doi:10.1002/prot.21139.
- Zhou Y, Frey TK, Yang JJ. . Cell Calcium. July 2009, 46 (1): 1–17. PMID 19535138. doi:10.1016/j.ceca.2009.05.005.
- Nakayama S, Moncrief ND, Kretsinger RH. . J. Mol. Evol. May 1992, 34 (5): 416–48. PMID 1602495. doi:10.1007/BF00162998.
- Hogue CW, MacManus JP, Banville D, Szabo AG. . J. Biol. Chem. July 1992, 267 (19): 13340–7. PMID 1618836.
- Bairoch A, Cox JA. . FEBS Lett. September 1990, 269 (2): 454–6. PMID 2401372. doi:10.1016/0014-5793(90)81214-9.
- Finn BE, Forsén S. . Structure. January 1995, 3 (1): 7–11. PMID 7743133. doi:10.1016/S0969-2126(01)00130-7.
- Stathopulos PB, Zheng L, Li GY, Plevin MJ, Ikura M. . Cell. October 2008, 135 (1): 110–22. PMID 18854159. doi:10.1016/j.cell.2008.08.006.
- Nelson MR, Thulin E, Fagan PA, Forsén S, Chazin WJ. . Protein Sci. February 2002, 11 (2): 198–205. PMC 2373453
. PMID 11790829. doi:10.1110/ps.33302.
外部链接
- Nelson M, Chazin W. . Vanderbilt University. [2009-08-29]. （原始内容存档于2009-08-25）.
- Haiech J. . European Calcium Society and the Université Libre de Bruxelles. [2009-08-29]. （原始内容存档于2009-08-09）.
upon request to haiech@pharma.u-strasbg.fr
- Yang J. . Georgia State University. [2009-08-29]. （原始内容存档于2009-10-12）.
prediction server for EF-hand calcium binding proteins
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.