phosphorylase, glycogen; muscle (McArdle disease, glycogen storage disease type V)
Myophosphorylase[1]
Identifiers
SymbolPYGM
NCBI gene5837
HGNC9726
OMIM608455
RefSeqNM_005609
UniProtP11217
Other data
EC number2.4.1.1
LocusChr. 11 q12-q13.2
Search for
StructuresSwiss-model
DomainsInterPro

Myophosphorylase or glycogen phosphorylase, muscle associated (PYGM) is the muscle isoform of the enzyme glycogen phosphorylase and is encoded by the PYGM gene. This enzyme helps break down glycogen (a form of stored carbohydrate) into glucose-1-phosphate (not glucose), so it can be used within the muscle cell. Mutations in this gene are associated with McArdle disease (GSD-V, myophosphorylase deficiency), a glycogen storage disease of muscle.[2]

Myophosphorylase comes in two forms: form 'a' is phosphorylated by phosphorylase kinase, form 'b' is not phosphorylated. Form 'a' is de-phosphorylated into form 'b' by the enzyme phosphoprotein phosphatase, which is activated by elevated insulin.

Both forms 'a' and 'b' of myophosphorylase have two conformational states: active (R or relaxed) and inactive (T or tense). When either form 'a' or 'b' are in the active state, then the enzyme converts glycogen into glucose-1-phosphate.

Myophosphorylase-b is allosterically activated by elevated AMP within the cell, and allosterically inactivated by elevated ATP and/or glucose-6-phosphate. Myophosphorylase-a is active, unless allosterically inactivated by elevated glucose within the cell. In this way, myophosphorylase-a is the more active of the two forms as it will continue to convert glycogen into glucose-1-phosphate even with high levels of glycogen-6-phosphate and ATP. (See Glycogen phosphorylase§Regulation).

Structure

PYGM is located on the q arm of chromosome 11 in position 13.1 and has 20 exons.[2] PYGM, the protein encoded by this gene, is a member of the glycogen phosphorylase family and is a homodimer that associates into a tetramer to form the enzymatically active phosphorylase A. It contains an AMP binding site at p. 76, two sites involved in association of subunits at p. 109 and p. 143, and a site believed to be involved in allosteric control at p. 156. Its structure consists of 24 beta strands, 43 alpha helixes, and 11 turns. PYGM also has the following modified residues: N-acetylserine at p. 2, phosphoserine at p. 15, 2014, 227, 430, 473, 514, 747, and 748, and N6-(pyridoxal phosphate)lysine at p. 681. There is a post-translational modification in which phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.[3][4][5] Alternative splicing results in multiple transcript variants.[2]

Function

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. This gene, PYGM, encodes a muscle enzyme involved in glycogenolysis. PYGM has a cofactor, pyridoxal 5'-phosphate, that aids this process. PYGM is located in the cytosol, extracellular exosome, and the cytoplasm. Highly similar enzymes encoded by different genes are found in liver and brain.[2][4][5]

Catalytic activity

Glycogen phosphorylase catalyses the following reaction:[4][5][6]

((1→4)-alpha-D-glucosyl) (n) + phosphate = ((1→4)-alpha-D-glucosyl) (n-1) + alpha-D-glucose 1-phosphate

Clinical significance

A myophosphorylase deficiency is associated with Glycogen storage disease type V (GSD5), also known as "McArdle disease".

A case study suggested that a deficiency in myophosphorylase may be linked with cognitive impairment. Besides muscle, this isoform is present in astrocytes, where it plays a key role in neural energy metabolism. A 55-year-old woman with McArdle disease has expressed cognitive impairment with bilateral dysfunction of prefrontal and frontal cortex. Further studies are needed to assess the validity of this claim.[7]

Additionally, mutations in the genes for myophosphorylase along with deoxyguanosine kinase have been associated with muscle glycogenosis and mitochondrial hepatopathy. The G456A PYGM mutation and duplication in exon 6 of dGK that results in a truncated protein have been associated with phosphorylase deficiency in muscle, cytochrome c oxidase deficiency in liver, severe congenital hypotonia, hepatomegaly, and liver failure. This expands on the current understanding of McArdle disease and suggests that this combination of mutations could result in a complex disease with severe phenotypes.[8]

An autosomal dominant mutation on the PYGM gene impairs activity of myophosphorylase-a, but not myophosphorylase-b. Symptoms include adult-onset muscle weakness and muscle biopsy shows accumulation of the intermediate filament desmin in the myofibers. Unlike McArdle disease (GSD-V, myophosphorylase deficiency), this disease does not have exercise intolerance since glycogenolysis is still possible through allosteric AMP activation of myophosphorylase-b.[9]

Interactions

PYGM has been shown to have 64 binary protein-protein interactions including 21 co-complex interactions. PYGM appears to interact with PRKAB2, WDYHV1, PYGL, PYGB, 5-aminoisatin, 5-nh2_caproyl-isatin, PHKG1, PPP1CA, PPP1R3A, DEGS1, SET, MAP3K3, INPP5K, PACSIN3, CLASP2, NIPSNAP2, SRP72, LMNA, TRAPPC2, DNM2, IGBP1, SGCG, PDE4DIP, PPP1R3B, ARID1B, TTN, INTS4, FAM110A, TRIM54, TRIM55, WWP1, AGTPBP1, POMP, and CDC42BPB.[10]

See also

References

  1. ↑ "RCSB Protein Data Bank - Structure Summary for 3MSC - Glycogen phosphorylase complexed with 2-nitrobenzaldehyde-4-(beta-D-glucopyranosyl)-thiosemicarbazone".
  2. 1 2 3 4 "PYGM glycogen phosphorylase, muscle associated [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2018-08-31.Public Domain This article incorporates text from this source, which is in the public domain.
  3. ↑ Carty TJ, Graves DJ (July 1975). "Regulation of glycogen phosphorylase. Role of the peptide region surrounding the phosphoserine residue in determining enzyme properties". The Journal of Biological Chemistry. 250 (13): 4980–5. doi:10.1016/S0021-9258(19)41265-9. PMID 1150650.
  4. 1 2 3 "PYGM - Glycogen phosphorylase, muscle form - Homo sapiens (Human) - PYGM gene & protein". www.uniprot.org. Retrieved 2018-08-31. This article incorporates text available under the CC BY 4.0 license.
  5. 1 2 3 "UniProt: the universal protein knowledgebase". Nucleic Acids Research. 45 (D1): D158–D169. January 2017. doi:10.1093/nar/gkw1099. PMC 5210571. PMID 27899622.
  6. ↑ "Reaction participants of glycogen phosphorylase". www.rhea-db.org. Retrieved 2020-12-26.
  7. ↑ Mancuso M, Orsucci D, Volterrani D, Siciliano G (May 2011). "Cognitive impairment and McArdle disease: Is there a link?". Neuromuscular Disorders. 21 (5): 356–8. doi:10.1016/j.nmd.2011.02.013. PMID 21382715. S2CID 36805481.
  8. ↑ Mancuso M, Filosto M, Tsujino S, Lamperti C, Shanske S, Coquet M, Desnuelle C, DiMauro S (October 2003). "Muscle glycogenosis and mitochondrial hepatopathy in an infant with mutations in both the myophosphorylase and deoxyguanosine kinase genes". Archives of Neurology. 60 (10): 1445–7. doi:10.1001/archneur.60.10.1445. PMID 14568816.
  9. ↑ Echaniz-Laguna A, Lornage X, Edelweiss E, Laforêt P, Eymard B, Vissing J, Laporte J, Böhm J (2019-10-01). "O.5A new glycogen storage disorder caused by a dominant mutation in the glycogen myophosphorylase gene (PYGM)". Neuromuscular Disorders. 29: S39. doi:10.1016/j.nmd.2019.06.023. ISSN 0960-8966. S2CID 203582211.
  10. ↑ "64 binary interactions found for search term PYGM". IntAct Molecular Interaction Database. EMBL-EBI. Retrieved 2018-09-05.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

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